Influência do consumo de adoçantes em parâmetros bioquímicos, histopatológicos e na biodistribuição da 99mTc-fitato de sódio em ratos Wistar.

Abstract

A diet rich in carbohydrates can cause some metabolic disorders as diabetes Mellitus. Aiming controle weight gain and plasma glucose, was launched in thet sweeteners. Teenagers usually consume sweeteners contained in diets and lights seeking good shape products. Often this use is uncontrolled and may cause metabolic changes. This study aimed to evaluate the effect of supplementation of an artificial sweetener (sucralose) and natural (fructose) on the metabolism of teenager rats, hoping to establish a comparative analysis between them. Eighteen male Wistar rats were used at 35 days of life. These animals were divided into 3 groups : control group (GC ) treated with distilled water and fructose group (GF) treated with 50mg/kg of fructose (natural sweetener) and sucrolose Group ( GS ) 50mg/kg sucralose during 24 days. The weight and feed intake were measured weekly. After the end of the administration, the weight and feed intake were measured weekly. Followings biochemical analyzes were measured: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, Gamma glutamyl traspepitidase (GGT), total bilirubin, direct and indirect bilirubin, total cholesterol and fractions (LDL and HDL), triglycerides (TG), albumin were performed and globulin), histopathological liver and % ATI /g radiotracer 99mTc-sodium phytate in liver and blood to verify the possible metabolic effects caused by these products. We used the ANOVA considering p <0.05 statistically significant. The GF showed higher body weight only in the first week compared with GS and GC (P<0.05). Histopathology and % ATI /g radiotracer 99mTc-sodium phytate in liver and blood did not differ between the groups. The GF showed higher values of AST, bilirubin, alkaline phosphatase and GGT compared with the other groups (CG and SG) (P< 0.05) . Analyzing enzyme ALT and albumin GF also had significantly greater difference, but only when compared with GS ( P < 0.05) . For other parameters, no statistical difference was observed. We conclude that the use of fructose during the experiment, was able to alter liver enzymes, but his well-known effect in increased triglycerides was not seen in the present study. Have sucralose caused no change. Thus, we see a need for a more prolonged study on consumption using moderate doses, and that this is also carried out with human recommendations to become more accurate and safer not only for adults but also for teenagers.