Avaliação de metodologia analítica para determinação de glicose e cloreto de sódio em soros.

Abstract

The present study aimed to evaluate a methodology to quantify the amount of glucose and sodium chloride in serum for greater patient safety and especially for control of quality. The saline and dextrose sera are best known by the population, although there other types of serum. The glucose solution for injection contains at least 95.0% and not more than 105.0% of the declared amount of C6H12O6, as a colorless sterile solution of anhydrous glucose or glucose monohydrate in water for injection. It contains no antimicrobial agents. The saline solution consists of sodium chloride and 0.9% using distilled water as a vehicle. Has a salt concentration in water equal to the cells, so called isotonic. This study aims to determine the glucose content in the serum glycosylated 5% using an enzymatic kit laboratory, and chloride in physiological serum used in the municipality of Cuité - PB using the technique of Molecular absorption spectrophotometry and Volumetry of rainfall, respectively. The methodology involved collecting samples of serum glucose and physiological used by Central Hospital and major pharmacies in the city of Cuité and quantification of the glucose content of the samples was performed by Molecular Absorption Spectrometry by Default External and quantification of the amount of sodium chloride by Volumetry Precipitation applying statistical parameters of the data collected. The development of this study contributes to verify that the levels of glucose in dextrose and sodium chloride in physiological saline study are consistent with the values actually labels on packages. During the entire study it was found very close to concentrations of expressed by manufacturers on the labels of bottles of each brand.