Análise in silico da variabilidade genética do oncogene E5 do HPV 16.

Abstract

The human papillomavirus (HPV) is largely responsible for causing several types of cancer, especially cervical cancer, and genital lesions, being the oncogene E5 belonging to this pathogen important for its oncogenic potential. This work analyzed the in silico genetic variability of gene E5 of HPV 16 gene sequences using the National Center for Biotechnology Information (NCBI) genomic database in order to identify, through the alignment of the codons, structural changes in the organization of the secondary structure of oncoprotein, which could influence its degree of pathogenicity. Analysis of HPV16 oncogene E5 variations deposited in the databases revealed the existence, through the PSIPRED software, of several polymorphic sites, by investigating the secondary structure of the protein, as well as the variations found in the variant sequences, with respect to the prototype sequence K02718. Regarding the mapping of the epitopes according to the IEDB platform standards, it was observed that the oncoprotein 16E5 sequence of the reference sample K02718 formed immunogenic epitopes with molecules belonging to MHC-I (HLA-A and HLA-B) and MHC- II. (HLA-DR), where HLA-A complex molecules are more related to the action of oncorprotein 16E5. Non-synonymous mutations promote changes in the secondary structure structure of 16E5 oncorprotein requiring a cell study to determine the impact that such changes have on the progression of carcinogenesis. The study of immunogenic epitopes is very useful for planning the identification and identification of early carcinogenic progressions, being computational biology a new strand of scientific study for identification and initial action for the development of immunotherapeutic strategies.