Avaliação toxicológica do pólen de oiticica (Licania rigida Benth) em abelhas (Apis melífera L.) africanizadas.

Abstract

The genus Apis is composed of four species and several subspecies are among those already living in South America. before arriving in Apis mellifera scutellata, and this originates in Africa. On the banks of rivers and in low places, there are naturally occurring Oiticica (L/cara/a ng/da) plant frequented by honeybees (4p/s me///Hera). This species is known by beekeepers because they reach at least one tablespoon clear honey crop very attractive for the consumer market during the offseason of myrtle. The flowering period of Oiticica is from August to October, with peak flowering in September, where beekeepers even make two or three crops of honey. Fernandes et al. (2006) also found that 1.. dg/da is a honey plant of great importance to beekeeping by Northeastern flourish in tomes of food shortages in the semiarid region. The collection and identification of pollen from flower oiticica was made in UFCG campus Pombal. In the Laboratory of Entomology, withdrew from the calyx and corolla of the flowers and then led to the drying oven at 40 ' C for 48 hours, soon after turned into powder by grinding in a mortar and sieved unto three meshes fine nylon, packing in plastic tubes, properly labeled. To assemble the bioassays were captured bees in the apiary beekeepers in the region, where the workers selected in the comb brood were newly emerged, ie the size and a lighter calor and taken to the laboratory in a plastic bottle (small boüle and cut fiam for ventilation). During the bioassays. the control group received only the candy and water, and insects of the treatment received the candy with the pollen of myrtle. Pollen was weighed into eight distinct segments, namely, (0.25% 0.50% 0.75% 1.0% 2.5%. 5%, 7.5% and 10%) and added to the "Candy" (mixture of icing sugar and honey in proportion 5:1). Placed in small plastic caps with a small screen wire covering to prevent the insect drown when the diet is liquid. Then distributed to a set of 20 insects per wooden box measuring 1 1 cm long by width and 1 1 cm high and 7 holes in the sedes closed with nylon fabric for ventilation. previously covered with filter paper and glass covers , along with a plastic cover with water soaked cotton swab. These distributed in three replications and control, making up 27 boxes and 540 worker bees tested. Acondicionaram up boxes in an environmental chamber with temperature set at 32 o C and humidity of 70%, where every 24 hours were removed from the BOD. observed, added water in cotton and then washing the dead bees. Upon completion of the bioassay, the data were placed on Bio Stat 5.0 program to do the calculations and preparation of graphics. on the result. For data analysis test was used by Log Rank Test method Collet, comparing the survival curves. We conclude this paper that the statistical analysis of data showed significant differences between treatments and control. therefore, suggests a toxic effect of pollen from flowers of Oiticica (l./can/a rfg/da Benfh) in workers of Após mellifera, the concentrations of 5%, 7 5% and 10%.