Análise de proteínas e carboidratos no fígado de Leptodactylus macrosternum (ANURA: Leptodactylidae).

Abstract

Anurans feature adaptive characteristics intrinsic to the group, they exert an important ecological function in the control of insects and other invertebrates, in addition, they are one of the most sensitive groups of environmental variation. The Leptodactylidae family is one of the most threatened and among the causes of decline is habitat reduction and climate change. In areas of Caatinga domain, the rain distribution pattern determines the energy activity of Anuran amphibians. Thus, the Leptodactylus macrosternum is considered an environmental bioindicator, which provides information on possible disturbances and conservation of ecosystems. Through this, this research aims to explore and contribute to the record of information about the quantification of proteins and carbohydrates present in the liver of the species, enabling better understanding Pós-quantificação. The place of collecting for the present study was in the Horto Florestal Olho D’água da Bica, located in the municipality of Cuité-PB, where 32 adults were used, collected fortnightly between the months of January to December of 2013. For the realization of this experiment, small liver fragments were removed, heavy, measured and routed to the histological routine. The carbohydrates were stained with PAS reaction and the proteins were stained with xilidina. In histological analyses in the liver, the presence of Kupffer and hepatocytes cells were observed. The quantification of proteins and carbohydrates were statistically analyzed. The monthly values of the quantities of proteins presented the smallest significant amounts during the month of July and the greatest values observed for this parameter were in the months of August and December. However, the quantities of carbohydrates did not present significant differences during the sampled months and the month of June was the one that presented the highest average values for this parameter followed by the month of May. There were only significant relationships between rainfall and the quantities of carbohydrates, in addition to temperature and quantity of carbohydrates. The variation in the rainfall and temperature regime has presented no significant relationship with the quantity of proteins deposited in the liver of L. macrosternum.