Estudo da leptospirose em pequenos ruminantes no Bioma Caatinga.

Abstract

Leptospirosis is a disease of a zoonotic character, which has a wide worldwide distribution, was identified as one of the most frequent illnesses that cause reproductive disorders in small ruminants. The objective of the work was to determine the frequency of seropositive female goats and male sheep to search for antiLeptospira spp. Antibodies, as well as to identify the predominant serogroups in the Semiarid of Paraiba, to detect Leptospira spp DNA. and bacteriological isolation of Leptospira sp., in the urinary tract of female goats and male sheep slaughtered in the public slaughterhouse in Patos, PB. 34 adult females of the caprine species were described in Chapter I and 24 adult males of the sheep species in chapter II. Samples of kidney and bladder tissue were collected in the slaughter line for later molecular detection through polymerase chain reaction (PCR) and bacteriological isolation. The samples with the best response were amplified and subjected to genetic sequencing. The blood collected was used for serological investigation using the microscopic agglutination test (SAM), using 24 serovars as antigens and a cutoff point of 1: 100. In Chapter I, the frequency of seroreactivity found was 17.6%, with titers ranging from 100 to 200. The serovars Autumnalis, Icterohaemorrhagiae, Tarassovi were the most frequent in this study by 33.3% each. In PCR, leptospiral DNA was found in 17.6% of renal tissue samples and 5.8% of the bladder. It was possible to perform the sequencing in a sample of renal tissue with 99% similarity to L. interrogans and isolation in two samples of renal tissue (5.8%) and one of the bladders (2.9%). In Chapter II, no seroreactive animal was found. In PCR, leptospiral DNA was found in 16.0% of the renal tissue samples and 41.6% of the bladder. It was possible to perform the sequencing in three selected renal tissue with 99% similarity to L. interrogans and isolation in two samples of renal tissue (8.3%) and two of the bladder (8.3%). We conclude that the urinary tract is a necessary site for the colonization of leptospires in small ruminants raised in the Northeast semiarid. The molecular results show a large number of asymptomatic animals with leptospires, require to associate MAT and PCR for a safe diagnosis of leptospirosis in goats and sheep.