Avaliação cicatricial in vitro de membranas de quitosana / Cissus verticillata (L.) Nicolson & C. E. Jarvis.

Abstract

Chitosan and Cissus verticillata (L.) Nicolson & C.E. Jarvis have physico-chemical and biological properties with therapeutic potential for repairing skin wounds. The objective of this study is to evaluate the healing potential of chitosan membranes with or without incorporation of plant extract, by applying it to 60 male Wistar rats, weighing between 250 and 300g, from the Cuité campus of the Federal University of Campina Grande (UFCG), as approved by the ethics committee the use of animals. To this end they were randomly divided into 4 groups, Control, MQ 0%, MQ 5% and MQ 20% in times of 7, 15 and 30 days. Once the biomaterials were implanted and the pre-established times were fulfilled, the tissues were removed en bloc, processed, stained with masson's trichrome and analyzed under light microscopy, evaluating inflammatory infiltrate, vascularization, healing, collagenization, fiber staining intensity, edema, tissue necrosis and multinucleated giant cells. The results showed the presence of an intense inflammatory infiltrate for the MQ 0% and MQ 5% groups compared to the Control in 7 days and mild / moderate for MQ 20%; vascularization induced until the 15th day for all groups, with subsequent decline; complete healing for MQ 20% with expressive collagenization in 7 days; and edema, tissue necrosis and multinucleated giant cells as non-significant events in all groups and times evaluated. It is concluded that the tested biomaterials played a significant role in inducing tissue repair, modulating the inflammatory reaction and acting in the reepithelization of skin wounds.