Helicobacter pylori em cães com leishmaniose.

Abstract

The presence of microorganisms with potential zoonotic as example the helicobacter in pets has been increasingly studied. This study aimed to investigate the presence of the gender H. pylori and possible changes in the gastric mucosa of dogs with leishmaniasis through different laboratory techniques, without previous history of gastric pathology. Therefore, six stomachs of dogs of different age, race and sex, diagnosed with Leishmania, were investigated through lymph node puncture, bone marrow and ear tip scarification. After euthanasia stomach fragments and swabs with stomach content were collected for histopathologic evaluation of the possible changes caused by the agent. For the identification of H. pylori were carried out rapid urease tests, microbiological culture followed by biochemical tests with the urease tests, catalase, oxidase, and nitrate reduction. On the rapid urease test, only two animals were negative form to the test in the pyloric region of microbiological culture, colonies suggestive for Helicobacter were subjected to biochemical tests for the characterization of the agent, three animals of the six showed biochemical characteristics of H. pylori. In histopathologic evaluation, the major changes in the samples stained with hematoxylin and eosin were inflammatory infiltration (lymphoplasmocytic) in the lamina propria, ranging from mild to moderate, commonly observed changes, but without an associated cause. Based on these results, we can conclude that dogs with Leishmania are susceptible to natural infection by H. pylori. Additionally, it is recommended that animals positive for Leishmania which are to undergo treatment should have their stomachs evaluated endoscopically and have samples collected for the diagnosis of H. pylori by biochemical methods and/or microbiological culture.