Frequência de anticorpos e tentativa de isolamento de Leptospira spp. a partir do trato geniturinário de suínos abatidos na microrregião de Catolé do Rocha, Estado da Paraíba, Brasil.

Abstract

Leptospirosis is an infectious disease that affects domestic and wild animals, and is an important zoonosis. In pigs we cause problems like abortions, weak milk and infertility, leading to several economic damages for breeders. Based on the absence of studies on a presence of bacteria in the animals of the region, the present study aimed to verify the frequency of anti-Leptospira spp. In addition to insulation. Samples of blood, renal fragments and vaginal secretions were collected from 50 slaughtered pigs without a municipal slaughterhouse in Brejo do Cruz between August 2016 and April 2017. The serological diagnosis performed by the microscopic serum agglutination (MAT) technique, using a collection of antigens Including the 24 serovars. For isolation as samples of renal fragment and vaginal secretion were collected in the STAFF culture medium and repixed to the EMJH medium, incubated in an oven and monitored weekly for 12 weeks. Of the 50 pigs examined, 20 presented anti-Leptospira spp. Agglutinins. A frequency of 40% was identified. Among the 20 seropositive 10 (50%) went to the serovar Tarassovi, and 5 (25%) to Bratislava, still reacted to Pomona, Bataviae and Icterohaemorrhagiae. In the attempt of isolation and growth in months of samples. The results suggest a low participation of other hosts in the transmission of leptospires to pigs in the region, and warn of a concern with preventive measures such as: vaccination of pigs, cleaning and disinfection of the facilities, proper destination of the pigs' waste, the use of gloves And boots for handlers, farmers and other people exposed to direct contact with these animals; Avoid contact with waste and water from floods and sewage, and among other measures with the aim of minimizing the risks of the occurrence of disease in the farms between the animals and their transmission to humans.