Levantamento da qualidade bromatológica e microbiológica de rações para cães.

Abstract

The objective of this study was bromatological and microbiological analysis in diets for puppies and adults. For chemical analysis, samples were packed in plastic containers, properly identified by letters and sent to the laboratory of animal nutrition for determination of crude protein (CP), ether extract (EE), mineral matter (MM), moisture, crude fiber (FB), calcium (Ca) and phosphorus (P). For the analysis of aerobic and mesophilic bacteria were pipettes 1ml aliquots of each of the three dilutions to Petri dishes (100x20 mm) sterilized, making each dilution in duplicate plates. For total and fecal coliforms, anaerobic microorganisms optional lactose fermenters with production of acid and gas within 24 to 48 hours of incubation at a temperature of 32 to 37oC, the methodology used to seriate tubes. For salmonella, gram-negative enterobacteria, and pipettes were added 1ml of dilution in medium sheets, coated in the culture medium dry, with a low film covered by a screen. For the survey of fungi in the diet using the method of counting total in Petri dishes. It was found that all the values declared in nutrition labels and observed in the analysis are overestimated in relation to the values recommended by the Ministry of Agriculture. All brands showed that 100% of fungal contamination and that the marks B, C and E had only bacterial colonies identified as total coliforms, not the presence to the other brands tested. There are brands tested in the presence of fungi and bacteria. The chemical analysis of feed showed different values for the nutritional composition of diets suggested by MAP. However, the diets tested are considered good because the level of contamination is below the limit set by law.