Estudo comparativo do efeito do plasma rico em plaquetas e do uso de gel cicatrizante fitoterápico em feridas cutâneas induzidas experimentalmente em equinos.

Abstract

Comparative study of the effect of platelet-rich plasma and the use of herbal healing gel on experimentally induced skin wounds in horses. The platelet-rich plasma may contribute to the repair process, reducing the time for tissue regeneration, because it is a natural source of growth factors. The proposition of this research was to compare the process of tissue repair in horses using plateletrich plasma gel and herbal healing. Two clinically healthy adult horses were used. The animals were used in two moments, repeating the same experimental protocol. Two experimental groups, group 1 (G1), treated with herbal healing gel, and group 2 (G2) treated with platelet-rich plasma, represented by each of the side edges of the forelimbs at the middle portion of the forearm were formed. For the experimental procedure, animals had both ends of the side members anesthetized with lidocaine and circular incision four inches in diameter were made. After the procedure dressings were made in both limbs. Group 1 (G1) received treatment with Septezan Healing Gel ® (commercial product), and group 2 (G2) received topical application of platelet-rich plasma. Wounds were assessed daily until the twenty-first postoperative as to edema, secretion, staining, presence and organization of granulation tissue and the presence of scar tissue. For histological evaluation of the healing process, biopsies of both experimental wounds (groups 1 and 2) at seven, 14 and 21 days postoperatively were performed. As a result, it was observed that both groups induced good healing process. In group 2 the presence of secretion occurred less intense. The appearance of granulation tissue was similar in both groups. In group 2, filling the wound with granulation tissue was faster and the organization and distribution in the area as evenly wound. There was no change in staining of wounds between groups. All animals in both groups showed edema, which on average lasted until the fourth postoperative day. Microscopically no differences between groups were found in the same periods. The results indicate that the platelet-rich plasma did not demonstrate superiority in the process of tissue repair in horses.