Potencial antimicrobiano e antiaderente do óleo essencial de Lavandula híbrida grosso contra cepas de Klebsiella pneumoniae.

Abstract

The use of products of plant origin in dentistry is an alternative in the prevention and combat of several pathologies that accompany humans. Klebsiella pneumoniae is a gram-negative bacterium that is closely related to nosocomial pneumonia because it has the ability to inactivate a large number of antimicrobial agents. Lavandula Híbrida, also known as Lavandin is a species derived from the union of Lavandula angustifolia Miller with Lavandula latifolia Linn. This species presents the varieties Abrialli, Grosso, Provence, Impress purple, and Super. Among them, the species that most demonstrated antimicrobial potential was the Grosso variety. Objective: to evaluate the antibacterial and nonstick activity of Lavandula Hybrid Grosso essential oil against strains of Klebsiella pneumoniae. Method: The assays were carried out using broth microdilution techniques in 96-well plates for determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) and sloped tube technique for the determination of Minimum Inhibitory Adherance Concentration (MICA) to the glass, in the presence of 5% sucrose. Results: It was obtained that the essential oil of Lavandula hybrida Grosso has a moderate antibacterial activity against the strains of K. pneumoniae seen by the MIC50 equal to ńŃ24 μg/ml, also possessing a bacteristatic potential. As to its anti-adherent activity, it was observed that the concentration of oil capable of inhibiting the adhesion of the microorganism to the wall of the whole was 1:32 whereas for the chlorhexidine digluconate 0,12% was 1:8. Conclusion: In accordance with the results of the present study, Lavandula Hybrid demonstrated to be effective as an antimicrobial and anti-adherent agent for the control and prevention of Klebseilla pneumoniae infection.