Análise da produção de metabólicos secundários por fungos endofíticos da Aloe Vera.

Abstract

Considering the pharmaceutical industry's need new substances that can give rise to new drugs, aiming to be more effective than the current ones and to have the ease of reproducibility of results, this work aims to analyze the production of secondary metabolites of endophytic fungi. from Aloe vera. Endophytic fungi inhabit the internal tissues of plant species and have great interaction with the host, such interaction can give them the ability to produce the same chemical substances originally characterized by the host plant, which is beneficial of pharmaceutical industry as it already has well-established techniques. large-scale production of substances from fungi. The choice of the plant species Aloe vera is justified by its ethnobotanical indication, which shows its soothing, moisturizing, healing and anticancer properties, among others. The plant parts were collected in the nursery on the campus of the Centro de Desenvolvimento Sustentável do Semiárido. Subsequently, the endophytic fungi were isolated in Potato-Dextrose-Agar medium containing Azithromycin. The fungi were cultivated for conidia production and then were re-cultured in oat medium, inducing the production of secondary metabolites. In turn, the different extracts obtained were analyzed using High Performance Liquid Chromatography technology, using a Reverse Phase column (HPLC-FR), resulting in 94.28% of the 35 secondary metabolites found having a hydrophobic molecular structure that, according to the literature, have a large antifungal and antiparasitic potential, supporting positive conclusions for possible obtaining substances with potential for the origin of new drugs.