Avaliação de técnicas de extração de DNA de abelhas sem ferrão (Frieseomelitadoederleini).

Abstract

Stingless bees have been victims of fragmentation in their natural habitats. The conservation of these species and their sustainable management is important to maintaining the Caatingaecosystem. In order to initiate studies of genetic variability in populations of native bees, this work aim to evaluate different methods of DNA extraction from thorax and wings ofF.doederleini, for being used in molecular phylogeny. The samples were collected in Santana do Seridó / RN and four protocols were tested: 1: Brazol ® reagent, dehydrated and frozen bees and 0.2% 2-mercaptoethanol in the lysis buffer; Protocol 2: Brazol ® reagent, dehydrated and frozen bees further 1μL of 2-mercaptoethanol into the samples; Protocol 3: fractionated silica, dehydrated and frozen bees, 0.2% 2-mercaptoethanol in the lysis buffer; Protocol 4: fractionated silica, dehydrated and frozen bees, further 2μL of 2-mercaptoethanol into thesamples. DNA extracted from dehydrated and frozen bees with Brazol ® was quantitatively and qualitatively superior to that obtained with silica.