Qualidade do leite UHT e identificação de Mycobacterium sp em bovinos com tuberculose e neoplasia pulmonar na Paraíba.

Abstract

Described in this thesis three papers related to the quality of UHT milk, diagnosis of tuberculosis and lung cancer associated with mycobacteria in cattle. In the first chapter, we evaluated the microbiological quality of UHT milk produced in Brazil and marketed in the State of Paraíba. For this they were analyzed 135 samples of 18 different brands and lots of whole and skim milk, and of these, 50 samples were selected for antibiotic residues. It was observed that 21.5% of the samples were contaminated with thermophilic, mesophilic and 16% by 8.1% by psichrophilic. No antibiotic residues were identified in the samples evaluated. There was no statistical difference in relation to non-standard samples as the criterion for assessing the frequency of milk production by region and by type of UHT milk for the studied bacteria. In the second chapter, it identified the presence of tuberculosis and M. bovis in cattle in the state of Paraiba, through various diagnostic methods. The macroscopic examination of the lesions was performed, histopathology, Ziehl-Neelsen (CZN), immunohistochemistry (IHC), bacteriology (CB), PCR and sequencing of the HSP65 gene (SG). All samples showed macroscopic lesions similar to tuberculoid granuloma, characterized microscopically by granulomatous inflammation, where the young animals had fewer injuries and large amount of inflammatory cells. Twelve animals were positive in the intradermal tuberculin test. The preview BAAR by CZN and the marking of the antibody in IHC occurred in 11 samples. Twelve samples were positive for M. bovis CB amplicaram and 11 samples of Mycobacterium gene by PCR. In a sample was isolated Mycobacterium avium in SG. The third chapter describes a case of pulmonary adenocarcinoma in a cow, associated with presence of Mycobacterium sp, isolated and identified. The epidemiological situation, clinical and macroscopic lesions were suggestive of tuberculosis, which was discarded after histopathology and confirmed its association by microbiological and molecular tests.