Isolamento e sensibilidade in vitro de Mycoplasma spp. e Mycoplasma agalactiae em pequenos ruminantes.

Abstract

Two studies were conducted to investigate the presence of Mycoplasma spp. in the reproductive tract of small ruminants and to test the in vitro susceptibility of isolates of Mycoplasma agalactiae. In the first study were collected 154 samples of vaginal and preputial swabs and semen from animals without reproductive disorders from four farms in the state of Paraíba. No lesions were observed in the external genitalia from studied animals. In the samples of vaginal swabs, two (1.3%) showed growth of Mycoplasma spp., being one in goat and other in sheep belonging to two farms. In the samples of semen and preputial swab were not observed Mycoplasma spp. growths. So it was confirmed the presence of mycoplasma in the reproductive tract of small ruminants from liquid and solid modified Hayflick’s media. For the second study, seven samples of Mycoplasma agalactiae were used, one sample from the previous study and six samples isolated from herds. The isolates were submitted to the antibiotic susceptibility test by the dilution plating method. The antibiotics used were: Tylosin (1μg/mL to 0,0625μg/mL), Enrofloxacin (1μg/mL to 0,0625μgg/mL) Oxytetracycline (0,5μg/mL to 0,03125μg/mL), Streptomycin (16μg/mL to 1μg/mL), Florfenicol (8/mL to 0,5μg/mL), Doxycycline (1μg/mL to 0,0625μg/mL), Gentamicin (1μg/mL to 0,0625μg/mL) and a biotherapic in formulation 30D. The enrofloxacin and tylosin were more effective at concentrations ranging from 0.25 to 0,0625μg/mL, oxytetracycline and doxycycline also had an inhibitory effect at concentrations from 0.125 to 0,03125μg/mL e 0,0625μg/mL, respectively. Streptomycin, florfenicol, gentamicin and the biotherapic showed no satisfactory inhibitory effects on the used concentrations. The enrofloxacin, tylosin, oxytetracycline and doxycycline showed better inhibitory effect on M. agalactiae growth.