Utilização do filme de quitosana na reparação de tendão em coelhos.

Abstract

This dissertation consists of a literature review of the various techniques of repair of tendon injuries and new surgical perspectives ( Chapter I) and an experiment that aimed to evaluate the effect of chitosan on the healing process of tendon tissue in rabbits ( Chapter II). We used 12 animals, six males and six females, New Zealand breed, weighing on average 3 kg, from the Experimental Farm Campus Banana UFPB, located in the municipality of Banana -PB. During the experiment, the animals were placed in cages and fed with commercial ration for rabbits twice a day and water ad libitum. Was instituted an adjustment period of seven days before surgery. The animals were randomly allocated into two groups of six animals each: a control group (CG) was removed a fragment longitudinal and transverse 1.4 cm of the Achilles tendon and the treatment group (TG ) after fragment removal tendon a movie chitosan was fixed over the defect generated with 4-0 nylon sutures. Each animal had both hind limbs operated. After 60 and 90 days after surgery, three animals from each group were euthanized for collection of materials and preparation of the slides for evaluation scar. The clinical evaluation was based on the presence of inflammation, infection and dehiscence of the suture. For histological evaluation was performed comparative study of the healing process through the cell type, amount of connective tissue and collagen fiber organization between groups and times. In the surgical wounds were not observed discharge or dehiscence. Histology comparisons between groups, the GC showed better healing process in relation to the GT at 60 days. At 90 days, the GT has outlined healing tendon recovery, with reorganization of cellularity and collagen fibers in connective tissue dense modeled. It was concluded that chitosan stimulates rapid cell growth, but haphazardly and complete healing only occurs after 90 days of its deployment in the tissue.