Avaliação In vitro da biocompatibilidade de membranas de quitosana / Cissus verticillata (L.) Nicolson & C. E. Jarvis.

Abstract

Chitosan and Cissus verticillata (L.) Nicolson & C.E. Jarvis present physico-chemical and biological properties with potential for therapeutic use in the treatment of skin wound repair. The objective of this study was to evaluate the tissue biological response in Wistar rats to the implantation of chitosan-based biomembranes associated or not with Cissus verticillata (L.). The tissue response was analyzed by histomorphometry in the subcutaneous tissue of the dorsal region of the rats. In the biocompatibility test, chitosan membranes were used without plant extract (MQ0%), with 5% of vegetal extract (MQ5%) and with 20% of vegetal extract (MQ20%) in times of 7, 15 and 30 days. The histopathological study considered for the analysis the inflammatory reaction (intensity of polymorphonuclear cells, mononuclear cells and multinucleated giant cells of the foreign body type) and the repair process (granulation tissue and young fibroblasts). The results showed significant for inflammatory infiltrate, granulation tissue and young fibroblasts. To the 7th day was moderate inflammatory infiltrate in all membranes at 15 ° days the granulation tissue is scarce for all MQ0% with reduction to MQ5% and on the 30th day. MQ20% presented moderate and scarce fibroblasts in 30 days for inflammatory infiltrate and granulation tissue. Thus, all membranes were considered biocompatible without causing abnormal tissue changes. It is concluded that the membranes modulate the inflammatory phase and stimulate the proliferation of fibroblasts, and thus, they can be applied to aid in the tissue repair process.