Ação antimicrobiana de solução à base de polihexametileno biguanida no tratamento endodôntico.

Abstract

The present study aims to compare the antimicrobial effectiveness of a solution based on polyhexamethylene biguanide (PHMB) and the action of chlorhexidine gluconate in root canal systems infected with Enterococcus Faecalis. Thirty-two human lower molars were selected and immersed in a test tube containing brain and heart infusion broth and sterilized in an autoclave, remaining for 24 hours at 37 ° C in a biological oven to confirm this procedure. Samples were contaminated with Enterococcus Faecalis and incubated for 4 weeks at 37 ° C, with culture medium renewed every 7 days. The teeth were divided into four groups: CHLx (n = 13) – Chlorhexidine Gluconate 2% gel, PHMB Group (n = 13) - 0.2% gel-based polyhexamethylene biguanide solution, Positive Control Group (n = 3) and Group Negative control (n = 3). Microbiological samples were collected with absorbent paper cones before (S1) and after (S2) the preparation of the distal canals with WaveOne Gold Primary instrument (25.07) associated with the analyzed antimicrobial substances. The total volume used to model each channel was 1.5mL of chlorhexidine or PHMB and 6mL of saline 0.9%. The results showed negative culture percentage in 77% of the samples for the CHLx and PHMB groups. According to the methodology used, it can be concluded that the use of gel substance CHLx 2% and PHMB 0.2% produced similar antimicrobial activity when used during root canal instrumentation.