Desenvolvimento e caracterização de membranas porosas de quitosana / Cissus verticillata (L.) Nicolson & C. E. Jarvis.

Abstract

This study aimed to develop and evaluate comparatively porous membranes of chitosan to different concentrations of the plant species Cissus verticillata (L.) Nicolson & C.E. Jarvis (insulin), in order to use in a controlled drug delivery system. It was realized data collection, botanical identification and the obtaining of the crude alcohol extract of the plant. For the production of chitosan and crude alcohol extracted chitosan membrane, it is used the solvent evaporation technique. The membranes were divided into groups: CM (chitosan membrane), CM5 (5% of crude alcohol extracted chitosan membrane) e CM20 (20% of crude alcohol extracted chitosan membrane), being the samples characterized by the techniques of Optical Microscopy (OM), Scanning Electron Microscopy (SEM), Traction Test and Cytotoxicity Test. The MO showed a change in color as the percentage of EAB increased, making the samples darker. The analyzes of OM and SEM revealed membranes with flat, regular surface, being possible to observe increase in the number of pores as the vegetal drug was incorporated. The mechanical tests revealed a decrease in tensile resistance when 5% of crude alcohol extract increased, and this increased to 20% of crude alcohol extract. The cytotoxicity test showed that all samples are viable for use in biological systems. Therefore, it was concluded that it was possible to develop porous chitosan membranes with different concentrations of crude alcohol extract, that the samples showed higher pores as the vegetal drug was incorporated; And that the CM group has a higher tensile resistance than the other groups.