Estudo filogenético e produção de marcadores moleculares in silico para diagnóstico de Fusarium oxysporum f. sp. rapae, in Ipomoea potatoes (L.) Lam.

Abstract

Sweet potato (Ipomoea batatas) is a vegetable of national importance, also it is the fourth most consumed vegetable in Brazil with great adaptation to different climates and soils and has a low production cost. Nationally, the south and northeast regions have the title of being responsible for the biggest part of the country's production, but currently the crops have been suffering several damages with the infection of fungal pathogens, especially the species Fusarium Oxysporum. The present work is intended to make a genetic study of the species Fusarium Oxysporum f. sp. rapae, that belongs to the Fusarium Oxysporum genetic complex, and also the production of primers for molecular diagnosis with the LYS2 gene. Researching genetic sequences using the blast tool of the NCBI platform, 15 sequences were selected to form the phylogenetic tree using the selected sequences and then design the primers for qPCR and the primers specific of the LAMP technique. The results obtained from the phylogenetic tree, with the bootstrap inference method, brought forward good results and similarities between species, resulting in a good tree of species with representation of the historical topology of the species. The primers designed with the primer3Plusplatform proved to be good primers, achieving all the parameters and only three of the five primer sets designed were selected for the LAMP technique. The developed protocols (in sílico) were based on molecular techniques to diagnose F. oxysporum in sweet potato, one protocol for qPCR technique and another protocol for LAMP technique.