Extração de celulase do bagaço de caju in natura e seu uso na hidrolise enzimática da palma forrageira.

Abstract

The objective of the present work is the extration of the celulase enzyme of the cashew bagasse in natura for its application in hydrolysis of the palm as cellulose source. The production of bioethanol using lignocelulósic biomass, in general, consists of three stages of operations: pre-treatment to separate cellulose and lignin, transformation of the cellulose in glucose for enzymatic or acid hydrolysis and alcoholic fermentation. For the stage of enzymatic hydrolysis of the cellulose celulase is used. The extration of celulase is influenced by factors such as: agitation, pH, temperature, ionic force of the medium, extraction time etc. In the present work the effect of variation of pH of the medium has been verified. After the extration of celulase the activity of the extract has been evaluated using the method of analysis of reducing sugars produced from the filter paper celulose. It was observed that the catalytic activity increased with the increase in the amount of filter paper used. The enzymatic hydrolysis of forrageira Palm using the cashew bagasse extract is favorable.The physicochemical characterization (pH, apparent density, humidity, ºBrix-soluble solids and reducing sugars) of cashew bagasse and the forrageira palm was carried out.