Modelagem estrutural de proteína OsJGSTF3 com potencial de desintoxicação de herbicidas em Oryza sativa japônica.

Abstract

Rice is an herbaceous plant in the grass family, from the genus Oryza, which feeds more than half the human population and therefore has great global importance. In spite of such importance, there are just a few studies to minimize the problems intrinsic to the cultivation of these cereals. One of the main limiting factor in the cultivation are rice weeds, due to competitor resources, inherent in a healthy development, the chemical control is still the must important form of weeds management, where in many cases the cultivar suffers productive losses, by contact with the herbicide. It is known that the superfamily of glutathione s-transferase (GSTs) enzymes confer on rice (Oryza sativa), a biotic and abiotic aesthetic, acts on biotransformation to protect against oxidative stress and excretion of xenobiotics, including herbicide detoxification. It is necessary to explore the mechanism of interaction, as well as the proteins with herbicides in question. A bioinformatics uses the molecular modeling method, for theoretical prediction of the structure of proteins, being a powerful alternative for overcoming some obstacles involved in the elucidation of protein structures by experimental techniques, since its celerity in the elucidation process and validation of protein structures in a short time and at reduced costs. This work aims to elucidate a three-dimensional/functional structure of a rice protein of a Glutathione Stransferase family and to validate it by in silico methodology, demonstrating its biotechnological importance and as advantages for agroindustry and agro-industry.