Avaliação da atividade antibacteriana e prospecção fitoquímica da resina da Albizia lebbeck.

Abstract

Albizia lebbeck is a tree, known in Brazil as faveiro or heart-of-black, endemic to India and can be found in Asia and Africa. One of the major characteristics of this species is its capacity for the production of resin containing bioactive compounds of biotechnological interest, being able to highlight the group of terpenes, widely used in the pharmaceutical and cosmetics industry. With this, present this work to identify the presence of secondary metabolites present in the exudate extract of A. lebbeck and to evaluate its antimicrobial activity. The pulverized resin underwent methanolic extraction and purification by means of HPLC on C18 reverse phase column. The chromatograms generated represented at least 20 different substances. These substances were divided into four distinct intervals of the chromatogram, with interval two (10min to 15min of retention) having the highest and best defined peaks. The study of antibacterial activity was carried out against the bacterial strains Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), with antibiotic Cloranfenicol being the positive control. There was inhibition of bacterial growth with about 1mm in diameter, with emphasis on Area 2, with halo formation for Gram positive and Gram negative. Phytochemical analyzes showed that Area 2 contains steroids and alkaloids. From these results, we suggest complementary studies that analyze the effectiveness of the antimicrobial activity of the tested extract.