Potencial de fungos da caatinga para produção de enzimas amilolíticas.

Abstract

The objective of the present study was to prospect fungi with potential for production of amylase from fungi isolated in the Paraiba (semi - arid region) and deposited in the Fungi Collection of the Center for the Sustainable Development of the Semi - arid / Federal University of Campina Grande (CDSA / UFCG) . This work represents a strategy to know the enzymatic diversity of organisms in this biome, as well as to apply them in future biotechnological processes. The analyzes were carried out from 50 isolates of this collection, of which only 27 showed growth in BDA medium and of these only 10 (ten) (37.04%) presented amylolytic activity. The determination of the enzymatic production was carried out in solid medium composed of agar and soluble starch, by means of the determination of the enzymatic index (IE). The highest IE values were obtained from the fungal isolates denominated CDSA 09, 18, 23 and 24 with values of 3.79 ± 0.5; 3.50 ± 0.5; 3.4 ± 0.4 and 3.5 ± 1.0, respectively. Fungal isolates CDSA 23 and CDSA 54 showed the highest growth rates, filling the entire Petri dish in 144 hours (6 days). The prospection of enzymes from microorganisms isolated from the Caatinga is of extreme value in order to reveal the microbial biodiversity of this region that is still little explored.