Produção e avaliação de antibiótico obtido por fermentação semissólida pelo fungo CDSA71.

Abstract

The emergence of increasingly drug resistant bacteria leads to the constant prospection and production of more effective, broad spectrum drugs, low toxicity and high specificity. The objective of the work in question was to evaluate the production of antibiotics from the fermentation process in semisolid medium, using filamentous fungi found in the Caatinga Biome, a microregion of Cariri - Paraíba. The fungus used in the experiment belongs to the genus Aspergillus, named CDSA71, obtained from the fungal collection of the Microbiology Laboratory of CDSA - UFCG, Sumé. The microorganism was inoculated in oat medium and after its growth the extraction of its metabolites was carried out by means of solvents. Then the metabolites were tested by means of the antibiogram to check the antimicrobial activity. The fungi were evaluated for their potential to produce antimicrobial compounds against Gram negative (Escherichia coli) and Gram positive (Staphylococcus aureus) bacteria. The antibiotic presented inhibitory halo of 2.42 cm and 2.25 cm in Escherichia coli and Staphylococcus aureus, respectively. After the antibiogram, it was verified that the metabolites produced by the fungus CDSA71 showed inhibition of the bacterial growth against the strains used.