Produção e caracterização da celulase (CMcase) por fungo isolado da fase termofílica de um processo de compostagem em fermentação em estado sólido tendo bagaço de coco verde como substrato.

Abstract

The agroindustrial activities have promoted an increase in the production of ligninolytic residues. Among the products of agroindustrial origin, it is possible to emphasize the green coconut bagasse, which has generated large amounts of residues due to increase of the green coconut processing for coconut water commercialization. The need for sustainable development has demonstrated the need for cleaner production. Thus, in this work the bagasse of the green coconut was used for the production of cellulolytic enzyme (CMCase), from a fungus isolated from the thermophilic phase of a composting process. The effect of temperature and humidity on the kinetics of CMCase production was evaluated in solid state fermentation using factorial design 22. The fungus used was identified as Aspergillus sp. and was able to colonize the green coconut bagasse and produce CMCase, and the best CMCase production was obtained at 45 ° C and 45% humidity (bu). The CMCase obtained in this condition presented optimum temperature and pH of 50 ° C and 5.0, respectively, and the half-life was estimated to be 17.5 minutes.