Cladistic analisys in geminiviridae: an evidence of multispecificity for cultivars hosts.

Abstract

In recent years the Geminiviridae family virus has been intensively studied due the severity of the diseases caused in several cultures of economic importance; like bean, cotton, corn, tomato and cassava. The objective of this work was to do a cladistic inference of Begomovirus populations through computational tools. Viral genome sequences were obtained from NCBI (https://www.ncbi.nlm. nih.gov/), totaling 297 sequences from different countries. The sequences were aligned with the Bioedit program, using the ClustalW algorithm. After the alignment, the cladogram was obtained through the Maximum Likelihood method in the MEGA program, with bootstrap analysis of 1000 replicates. ModelTest was used to select the most suitable evolutionary model (Reversible G + I) for the data set. The results showed that the bootstrap values in the clades ranged from 9 to 100. Pepper, potato and watermelon virus sequences were used with outgroup, but they were grouped into clades shared by the viruses obtained in the Tomato strain, revealing an evidence that the mosaic virus in these cultivars belong to the same species and same genus.