Avaliação da produção de celulase em mesocarpo de coco verde (cocos nucifera) por meio de fermentação semissólida por um fungo isolado de um processo de compostagem.

Abstract

The prospect of cellulase producing fungi is one of the possible strategies for achieving necessary enzymes to hydrolyze lignocellulosic materials. Therefore, composting tanks represent a potential site for the isolation of microorganisms capable of producing enzymes of industrial interest, such as cellulases. Cellulases are enzymes that excel in several areas, playing important roles as the hydrolysis of lignocellulosic material to obtain fermentable sugars for the production of third-generation fuels. The production of this enzyme in medium semisolid fermentation (FSS) has been extensively studied due to demand for higher enzyme production and thereby reducing the enzyme production costs. The objective of this study was to evaluate the production of cellulase (CMCase) by semisolid fermentation of green coconut mesocarp by a fungus isolated from a composting process. The study of the production of cellulases was carried out through the factorial design 22 with triplicate at the midpoint. The mesocarp of the coconut inoculated with the fungus 302 was evaluated for the production of cellulases with an initial moisture content of 45%, 55%, 65% and temperature range 30 ° C, 37 ° C and 45 ° C. The fermentation was conducted for 160 hours, during which we analyzed the capacity of the enzyme cellulase (CMCase), the effect of humidity and temperature by the fungus 302 and microbial growth. The peak enzyme production expressed CMCase (0.54 U.mL-1) occurred in 120 hours of fermentation, 65% and 30 ° C. When analyzed the growth of fungus and enzyme production it is noticed that the fungus could grow even without producing high enzyme values.