Produção de celulase por fermentação e estado sólido empregando resíduo de coco verde (Mesocarpo) sob a ação de um fungo isolado na fase termofílica de um processo de compostagem.

Abstract

The aim of this work was to produce cellulases and estimate the effect of humidity and temperature in the production of cellulase (CMCase) through solid state fermentation using two isolate fungi obtained from a composting process, and having grinded green coconut mesocarp as substrate. The study of CMCase production was made through the factorial design 22 with triplicate at the center point, and the statistical analysis was performed using Statistica TM software. The washed grinded green coconut mesocarp inoculated with Fungi F1C3 was evaluated for cellulase production with initial moisture contents of 45, 55 and 65% and temperatures of 30, 37 and 45°C. Fermentation was developed along 168 hours during which the parameters pH and enzyme activity expressed in CMCase were analyzed. For the Fungus F1C3, the peak production (CMCase) (0,6 μmol.mL-1.min) occurred in 72 hours of fermentation, 45% humidity and 45 ° C. Statistical analysis showed that the factors did not influence in enzyme production.