Bioprospecção de renina a partir de fungos filamentosos da caatinga.

Abstract

Proteases are one of the most important enzymes used in industrial processes, especially in the food industry, one of its main applications in the dairy industry, such as milk coagulant in cheese manufacturing processes. In order to optimize the area of dairy, new sources of coagulating enzymes are being researched, providing a search for new microbial strains producing these enzymes. The milk coagulation through microbial enzymes is one of the methods that has become more advantageous in allowing reduction of process costs and its large-scale production by fermentation. This study aimed to analyze the production of renin by Caatinga´s fungi and effectiveness of these enzymes in the milk coagulation. The renin production occurred via submerged fermentation and analysis of the coagulation activity was performed by milk coagulation tests. Supernatants from CDSA01 fungi, CDSA24 CDSA72 and grown in the presence of casein as a stimulant showed coagulant activity. The CDSA01 and CDSA24 showed better coagulant activity of 9.88 UAC / mL and 11.54 UAC / mL, respectively, at 24 hours and 96 hours of fermentation, since the CDSA72 showed better coagulant activity of all the supernatants of 16.17 AUC / mL in 72 hours fermentation.