Análise da sintetização da enzima invertase por fermentação em estado sólido utilizando resíduos agroindustriais.

Abstract

Invertase can be known also as β-fructofuranosidase D, and hydrolase, that can be detected in a wide variety of eukaryote and prokaryote organism. In the industry, it is the main enzyme used in the hydrolysis of sucrose, and also in obtaining the invert sugar syrup. For this enzyme production, the most widely way of fermentation in scientific production is the solid state fermentation. This study aimed to analyze the performance of the SSF in the invertase enzyme production, using the filamentous fungus Aspergillus niger IOC 4003 as an agent, using two waste (bran and rice bran mesquite) as a substrate. The fermentation took place in 250 ml Erlenmeyer flasks containing the inoculated medium with the chosen substrate, where the whole process is kept under incubation at room temperature. As regards the period of the whole process was seven days. For every 24 hours were collected a erlenmeyer flask containing the fermentation of each substrate. The extracted broth from each flask by vacuum filtration had each pH, substrate consumption, enzymatic activity, percent moisture and amount of crude protein analyzed. Cell growth was analyzed by dry weight, which took the difference of the weights eppendorf tubes containing the dried biomass and sterilized empty eppendorf tubes dried in an oven. According to this, the Aspergillus niger invertase IOC 4003 production was satisfactory by using of the solid state fermentation and the chosen residues. It was observed that in both fermentations, the invertase activity levels increased during the established period and shown to be directly proportional to the cell growth and productivity. Since the Bradford protein quantification levels shown to be consistent even with a fluctuation in fermentation using mesquite. The pH values maintained an acid character, allowing a good performance of the fungus. In this way, the invertase production by solid state fermentation provides a better more affordable viability, adding value to the waste that would be discarded, and strengthening the sustainability and economic viability of the enzyme production process.