Efeito de queijo caprino probiótico na ação antinflamatória intestinal e no perfil glicêmico de ratos colíticos.

Abstract

Studies have shown that diet has a great effect on the modulation of the intestinal microbiota, contributing to the balance between microorganisms and host. Modulation of the intestinal microbiota and immune response indicate a probable application of probiotic products in the treatment of Inflammatory Bowel Diseases as well as of the systemic diseases that accompany the inflammatory process, diseases that act directly on the alteration of the lipid and glycemic profile. This study aimed to evaluate the impact of administration of a rennet-like goat cheese added to Lactobacillus rhamnosus EM1107 on colonic damage and biochemical parameters of rats with induced colitis. Thirty-two rats of the winstar strain were divided into 4 groups: negative control (CN), colitic control (CC), probiotic cheese (QP) and sulfasalazine (SZ), all receiving water and ad libtum ration. The QP group received via gavage 2.5 mL of a solution containing 1 g of curd goat type added with Lactobacillus rhamnosus EM1107 (10-7 UFC / g). After 15 days of pre-treatment, the animals of the CC, QP and SZ groups were anesthetized for induction of colitis by means of a rectal catheter, in which 0.25 mL of 30 mg DNBS (dinitrobenzene sulfonic acid) solution was injected. On the fifth day after induction of colitis, the animals were anesthetized and sacrificed by cardiac puncture, with collection of serum samples for glucose, total cholesterol, HDL-cholesterol, triglycerides and LDL-cholesterol. The colón was collected for macroscopic assessment of colonic damage. As for the colonic damage score, a reduction was observed in the QP and SZ groups when compared with the CC. The animals receiving treatment (QP and SZ) presented lower values of weight and weight / length relation of intestinal colon, compared to the others, demonstrating a reduction in the macroscopic damage of the colitic lesion. Regarding the biochemical parameters, it was observed that the treatment with the probiotic product caused a reduction in the glycemic levels being in amounts similar to the animals that did not have injury. This shows the hypoglycemic effect. The same happened as the lipid profile, with reduction of serum cholesterol, LDL and triglycerides and increase of HDL. Goat's milk added from probiotic culture showed to have intestinal anti-inflammatory activity, hypolipidic and hypoglycemic effect when administered as a pretreatment in colitic animals.