Efeitos da suplementação da Spirulina platensis sobre parâmetros de ansiedade e memória na prole de ratas tratadas durante a lactação com diferentes tamanhos de ninhadas.

Abstract

Spirulina platensis (SP) is an algae. Contents in its composition a high content of protein, about 60-70%, also has carbohydrates, lipids, vitamins and minerals and other fotonutrientes. The objective of this study was to evaluate the effects of malnutrition multicarencial and supplementation of SP on anxiety parameters and memory of the offspring of mice with different size litters treated during lactation. Four groups of animals were formed: one Normal control group (Con N) treated with distilled water; a Regular Spirulina group (Spiru N), treated with 8% Sp solution; a Great control group (Con G), treated with distilled water; and a Grand Spirulina group (Spiru G), treated with 8% Sp solution, increasing the size was to induce a multicarencial malnutrition, by competing for nutrients. The animals received food and water ad libitum. The Sp solution was supplied by gavage at the ratio of 2ml/100g. To evaluate parameters of anxiety were performed the following tests: Test Field Open, whose parameters evaluated were ambulation, get up, grooming and defecation and Test of Elevated Plus Maze (EPM), which evaluated the number of entries and time of permanence in closed and open arms and head-dipping. To measure changes in memory were used: Habituation in the Open Field Test for assessment ambulation as a memory index and the facilitation and the Object Recognition Test, where were examined the short and long term memory. The data showed that the animals of the Spiru N demonstrated an increase in grooming compared to Con G. In the EPM the Spiru N and G entered less in the closed arms and Con G and Spiru G staying longer in the closed arms and performed less diving head (p<0.05). The test of habituation showed no statistical differences. The object recognition test in short term showed no statistical differences, but in long-term memory there was a further exploration of the new object compared to family in Con group N. This exploration of the new object was decreased in Con G group compared to the Con N and Spiru G (p <0.05). The model multicarencial malnutrition used in this study was efficient to induce increased anxiety and memory impairments in the long term in the offspring of female rats treated during lactation. More over, supplementation of lactating rats with Sp was not anxiogenic effective to reverse the damage, but to reverse the long-term memory related damage caused by competition of nutrients by increased litter size.