Aspectos histomorfométricos do fígado de Leptodactylus macrosternum coletados na região de Cuité, PB.

Abstract

The Leptodactylus macrosternum (ANURA, LEPTODACTYLIDAE), is an amphibious anuro of nocturnal and its preferred habitat lentic water bodies. The liver plays an important role in these animals in nutrient storage processes. Thus, this study aimed to analyze the morphologic aspects of Leptodactylus macrosternum liver. The livers are from animals collected from the city of Cuité-PB, which were fixed in formalin, remaining the same for 24 hours. Subsequently the body was cleaved to give fragments, which were then dehydrated in ethyl alcohol (increasing concentrations), diaphanized by xylene, and embedded in paraffin impregnated. In sequence, the sections were subjected to histological staining technique hematoxylin-eosin, analyzed in light microscope OLYMPUS BX-49 brand and photographed under light microscope OLYMPUS BX-50. The morphometric analysis of the liver, using a grid with 100 points-test was performed, placed on the cuts of histological slides stained with hematoxylin-eosin. Counting was done in prepared slides, which were counted 10 fields, using the 40x objective, a total of 5,000 points per group. Similarly, Kupffer cells on slides stained with hematoxylin and eosin were quantified. All data were analyzed using Kruskal-Wallis nonparametric test with Dunn's post hoc test (P <0.05). There was no significant variation in the population of L. macrosternum, nor correlation between the emergence of more males or females when compared to the rainy season, the values of liver somatic index did not show significant variations. Histological analysis describes the liver as being similar to most vertebrates. The analysis of data from histomorphometric Kupffer cells show significant variation among individuals only 4 of the blade compared with the blades 7, 9 and 11.