Seleção in sílico de genes miraculina de Citrus sinensis candidatos a ensaios funcionais.

Abstract

The study of miraculin gene expression in Citrus sinensis is of great importance, considering the financial importance of the citrus crop and studied varieties as well as the involvement of these genes in the development of the fruits and the responses of plants to biotic and abiotic stresses. The objective of this work was to select Citrus sinensis miraculin-lke genes of interest for functional studies using in silico analysis based on information from databases CAP and CitEST. The strategy employed involved the identification of genes in keyword by genomes, analysis of similarity via BLASTx, ORFs analysis, analysis of physical-chemical parameters and functional domains, analysis of the expression in organs and phylogenetic inferences from Multiple alignment of protein sequences. As a result, the genome of the plant species Citrus sinensis found 16 genes Mir identified in cv. "Valencia" and available in CAP database (Citrus sinensis Annotation Project). Ten miraculin genes from the species Citrus sinensis genotype cultivar 'Valencia' presented different sequences from those of genotype cultivate Pera selected in CitEST database. Of the 16 genes sweet orange Mir 'Valencia' identified, four were identical to four single genes cultivar Pera and have a high level of expression in fruits, leaves and flower under varying conditions. Of the four proteins deduced from C. sinensis selected and characterized molecularly, three presented domain to Trypsin Inhibitor superfamily of Kunitz. Of the four proteins deduced from C. sinensis selected, three presented theoretical pH acid and a base. CsMir1 and CsMir2 genes represent good candidates for molecular activity assays in silico, by presenting best molecular information from the analyzed viable for it to function at the level of expression, given their importance in biotechnological applications.