Estabelecimento de protocolos para microextração de DNA de plantas do semiárido.

Abstract

The Caatinga biome is located in semiarid climate region of Northeast Brazil and consists of vegetables xerophilos, as is the case of cacti (mandacaru and xique-xique). In the last decade has seen a growing interest in exploring the genetic resources of plants from semiarid region in order to understand mechanisms of resistance to drought, as well as the search for biomolecules involved in resistance to diseases caused by pathogens and pests. The objective from this work was to establish protocols for extraction of genomic DNA from Caatinga plant leaves. Then, three protocols were for extraction of genomic DNA from Caatinga plants were elaborated, tested and compared in 10 plant species native or introduced Caatinga, according to physiological characteristics of the species. Plant materials were collected in the Horto Florestal Olho D’Água da Bica, Health and Education Center CES/UFCG, located in the Cuité-PB and Line of Pear Site in Jacana-RN. As a result, it was possible to isolate genomic DNA from 10 plant species selected by the three methods compared, albeit with significant differences in efficiency on the quantity and quality of DNA isolated according to the estimation made in Nanodrop spectrophotometer. In general, the Protocols I and II produced the highest amounts of isolated DNAs for an equal number of species. However, Protocol I produced a larger amount of DNA of three of the four species that have tender leaves, whereas Protocol III produced a larger amount of DNA in four of the six maceration difficult species.