Identificação e caracterização In silico da expressão de genes do tipo Miraculina em citros.

Abstract

Brazil is considered the largest producer of citrus in the world currently holding about 26% of production and 53% orange juice, generating approximately $ 1.5 billion with annual exports of frozen concentrated orange juice. However, over the years, the citrus have suffered significant losses due to environmental factors and particularly pathological. Phytophthora parasitica, citrus variegated chlorosis (CVC), leprosis citrus and citrus sadness virus (CTV) are the major causative agents of disease in citrus. A strategy is currently exploring relevant databases to analyze the expression of genes involved in plant responses to pathogens in order to understand the biological functions of these genes and the direction of future work considering their biotechnological applications. The aim of this study was to identify and characterize the expression of genes present in citrus miraculin database CitEST, using computational tools to characterize and in silico miraculin gene expression in at least one citrus species found in CitEST. We searched for genes in citrus type miraculin in the database of functional genome of citrus - CitEST using Unigene Editor program and the keyword "miraculin" and identified the sequences were analyzed using BLASTx to find homologous sequences in miraculin GenBank database. We found 127 genes in the type miraculin database CitEST, which are expressed in organs of species Poncirus trifoliata, Citrus reticulata, C. sinensis and C. aurantium. Furthermore, there are miraculin genes with homology to proteinase inhibitor expressed in organs and Poncirus trifoliata induced under conditions of pathogens.