NASCIMENTO, J. P.; http://lattes.cnpq.br/5645028726118191; NASCIMENTO, Josefa Paula do.
Abstract:
Rhodotorula is associated with a variety of pathological processes in humans, in severe cases the mortality rate reaches 50%. The amphotericin B and fluconazole are the drugs of used in the treatment of infections Rhodotorula spp. however, some strains of the yeast already have degrees of resistance to fluconazole. The species Sida santaremnensis occurs in almost all Brazil, despite its wide distribution, there is no report of this kind of use in folk medicine. However, checking the variety of natural compounds, pharmacological properties and use reports to described species of the family Malvaceae, notably in the gender Sida, and to contribute to the development of new antifungal agents, we sought to assess by determining the antimicrobial activity by Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM) of crude ethanol extract (BSE) and fractions of hexane (HEX), dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and water-alcohol (ETOH:H2O) of S. santaremnensis about twelve strains Rhodotorula spp. The MIC determination of S. santaremnensis was performed by the microdilution technique, using 96 well plates. After performing microdilution determined to CFM. Assays were performed in duplicate and the results expressed as the geometric mean of the inhibitory concentrations obtained in two trials. It was found that the BSE and CH2Cl2 and EtOAc fractions inhibited three of the twelve strains tested. The MIC, where not visualized fungal growth was between 2048 μg/mL and 512 μg/mL. The extract and fractions that showed antifungal activity, also were behaved fungicide. The majority of strains of Rhodotorula spp. was not sensitive to the crude extract and fractions of S. santaremnensis, demonstrating that the development of antifungal the basis of this plant in the future, it would not indicated for treatment of infections Rhodotorulla spp.
