LINS, S. A. S.; http://lattes.cnpq.br/7329862411748916; LINS, Simone Aparecida da Silva.
Abstract:
Cellulases, among many enzymes, have been highlighted in several activities niche, such as
food, detergents, pharmaceuticals, textiles, pulp and paper and wastewater treatment of
effluents and residues. Currently, most commercially used microbial cellulases are produced
by the species Trichoderma, in particular, Trichoderma reesei with proven ability for the
production of cellulolytic enzymes. Studies have been developed in order to produce this
enzyme through a process of solid state fermentation from lignocellulosic agroindustrial
wastes, thus reducing the cost of enzyme production, and adding value to the residue. The aim
of this work was to produce cellulases from the stalk of the cashew bagasse using
Trichoderma reesei LCB 48. Characterizations were carried out aiming at the residue of the
chemical composition of the pulp relative to the cashew nutrients, since it was used as
substrate for the fermentative process for production of cellulase. The study of the production
of cellulase was performed using the 2 factorial design with central point in quadruplicate.
The stalk of the cashew bagasse washed inoculated with T. reesei was evaluated for the
production of cellulases with initial moisture contents of 45, 55 and 65% and in the presence
of inorganic nitrogen (ammonium sulfate) at concentrations 0.5, 0.75 and 1%. The
fermentation was developed over 238 hours during which the parameters analyzed were pH,
moisture, AR and enzyme activity expressed in CMCase. Through the characterization of the
waste bagasse from the stalk of the cashew confirm that this substrate has the potential to be
used in fermentation for production of cellulolytic enzymes, mainly because it presented a
satisfactory percentage of cellulose, for that is known to be an excellent inducer of these
enzymes. Peak production of cellulase enzyme expressed in CMCase was achieved with 238
hours this being with a value of 0.71 U/g (0,095 U/mL), under the conditions of 45% initial
moisture content and 1% of nitrogen source. Using the methodology of experimental design
allowed us to observe that the initial substrate moisture is a key variable in the production of
enzymes CMCases, and that the minimum level of humidity (45%) showed the highest
production values of CMCase.