Investigação do mecanismo de ação do Geraniol e do Citronelol contra Candida Albicans.

Abstract

Due to the increased incidence of fungal infections, especially Candida albicans, and the increasingly frequent reports of fungal resistance, it is necessary to search for new treatment options. Natural products represent a vast field of research, with many reports in the literature of substances of natural origin with potential antifungal, among these substances are essential oils and their components. This study aims to investigate the activity of citronellol and geraniol against C. albicans in order that these substances are components of some essential oils that have antimicrobial activity. Through the microdilution technique was determined the Minimum Inhibitory Concentration (MIC) of geraniol and citronellol; being that when an aliquot from each well of the microtiter plate where no growth was plated on Sabouraud Dextrose Agar (SDA) met the minimum fungicidal concentration ( CFM); further by microdilution technique, we investigated the ability of substances interacting with the ergosterol and interfering with the cell wall. The results showed that these substances have antifungal activity, the MIC of geraniol and citronellol was 250 mg / mL for all strains tested. The CFM of geraniol against strains ICB-12 and ATCC 76615 was 1000 mg / mL, against all other strains was 250 mg / mL. The CFM of citronellol against ICB-12 strain was 500 mg / mL, against the other strains was 250 g / mL.Na investigation of the mechanism of action was found that both geraniol as citronellol ligated to ergosterol, which can be observed by increasing the MIC. There was no increase in MIC of either oils in the presence of sorbitol, which suggests that the action of geraniol and citronellol not occur per share in the cell wall. The results show that these substances are potential antifungal agents, with strong antimicrobial activity, being proven by their considerable values of MIC and MFC, as to its mechanism of action by its binding to ergosterol, draw a profile of a possible target that offers selectivity in the action of these substances may be continued studies to detailed design of your facility as well as conducting preclinical studies clinicians.