FRANÇA, T. C. P. T.; http://lattes.cnpq.br/0748185051277328; FRANÇA, Tatiana Clementino Pinto Toscano de.
Resumo:
With the increasing number of cases of people with diabetes in recent years,
and in view of the existing biosensor technology still has a high cost-benefit
ratio, found himself in a race last decade the development of biosensors for
detection of glucose based on the immobilization of glucose oxidase mainly
using functionalized magnetic nanoparticles of magnetite agents silanes. Thus,
this dissertation developed a material capable of immobilizing glucose oxidase.
For this purpose it has been proposed to obtain a hybrid material from magnetic
nanoparticles (NPMs) magnetite (Fe304) surface modified with silane agent, 3-
aminopropyltriethoxysilane (APTES) .To assess the product obtained regarding
the effect of the silane agent in the modification of the properties of NPMs
analyzes were performed characterization of X-ray diffraction (XRD),
spectroscopy in the Fourier transform infrared (FTIR), thermogravimetric
analysis, scanning electronic microscopy (SEM) and magnetic measurements in
vitro test magnetic separation and sedimentation. To evaluate the interaction of
NMPs with and without silane agent test was performed immobilization of
glucose oxidase. The results obtained showed that the surface modification of
Fe304 NPMs silane agent APTES was successfully achieved since the
silanization process did not alter the characteristic of the structural and
morphological magnetic product. The magnetite samples before and after the
silanization were non-cytotoxic and it has high cell viability. The immobilized
glucose oxidase was obtained, with 0,006mg/g for pure magnetite and
0,008mg/g for the hybrid Fe304/Si02 . The type of chemical interaction (physical
adsorption or covalent) between the glucose oxidase (GOX) and NPMs, before
and after the immobilization, were influenced by silanization. These reveals that
magnetite and the hybrid Fe304 / S i 0 2 are carriers for GOX immobilization and
the immobilized GOX has great potential application in developing glucose
biosensors.