Potencial modulador de monoterpenos sobre a sensibilidade de dermatófitos a terbinafina.

Abstract

Dermatophytes are infections that affect the keratinized tissues such as hair, nails and horny skin extract of humans and animals, caused by fungi called dermatophytes. These fungi are divided into three genera: Microsporum, Trichophyton and Epidermophyton. Microsporum and Trichophyton are among the genera of dermatophyte fungi that cause tinea capitis (scalp). Terbinafine, an allylamine, is widely used in the treatment of dermatophytosis. However, the emergence of resistant fungi imposes studies with natural products among the monoterpenes, present in essential oils and presenting a diversity of biological activities, including antifungals. These may be presented as novel fungal sensitivity modulating agents by inhibiting plasma membrane flow pumps. In this study, the modulating activity of monoterpenes terpinolene and dihydrojasmone on the resistance of Microsporum canis LM 216, Trichophyton Interdigitale H6 e Trichophyton interdigitale Ämdr2 to terbinafine was analyzed. The minimum inhibitory concentration (MIC) of test drugs was determined by microdilution. The effects of test drugs on plasma membrane functionality (K+ release) were performed. Subsequently, a terbinafine MIC was determined at subinhibitory monoterpene concentrations (modulation assay). Finally, an association study was performed with terbinafine and monoterpenes (checkerboard). Dihydrojasmone was more potent than Terpinolene because it had lower MIC values (12 ìg / mL, 256 ìg / mL and 32 ìg / mL). All fungi used in termination from 1 ìg / mL. All test drugs increased K+ release (p <0.05), affecting plasma membrane functionality. Dihydrojasmone modulates the resistance of all strains tested in front of terbinafine; and Terpinolene models the resistance only of M. canis LM216 and T. interdigitale Ämdr2. The monoterpenes tested have an antifungal potential and a modulator of the sensitivity of the tested strains to terbinafine and the association between the drugs shown to be synergistic.