Efeito tóxico do pinhão branco sobre operárias de abelhas africanizadas Apis Mellifera L.

Abstract

The White Jatropha (Jatropha curcas L.) is a native species of the Euphorbiaceae family, demanding strong insolation and drought resistance which today presents no economic application. The present work aimed to study the toxic effect of macerated flowers Pinion White (Jatropha Curcas L.) on Após mellifera Africanized bees in a controlled environment. A collection of flower Pinion White was made near the Federal University of Campina Grande - UFCG campus Pombal. They were then brought to the drying oven at 40 ' C for 48 hours, followed by grinding in a mortar, then made unto powder and sieved into three meshes thin nylon, placed in plastlc tubes and properly labeled at the Laboratory Animal Nutrition UFCG - Pombal - PB and led to the Bee Laboratory UFCG - Pombas - PB. The macerated pinion White Flower was weighed unto three distinct fractions, namely (0.25 g, 0.50 g and 1.0 g) and added to an artificial diet known as "candy". Placed in small plastic caps with a small screen wire covering to prevent insect drown when the diet is liquid. Placed in wooden boxes measuring ll cm long x ll wide and 7 enches tala and holes in the sides closed with nylon mesh for ventilation, previously lined with filter paper and glass covers. The workers were selected in the brood comb the newly emerged, ie: by the size and a lighter color and taken to the laboratory in a plastic bottle (s.mail bottle and cut foam for ventilation). Then distributed set of 20 insects per box, with a plastic cover with water-soaked cotton swab. These distributed in three replicates and control, making an average of 12 boxes and 240 worker bees tested. Put an environmental chamber with temperature set at 32 ' C and 70% humidity. Every 24 hours are removed from the BOD observed water added with a syringe then withdrawing the dead bees recorded on a record control daily throughout the test period. The data were placed on BioEstat 5.0 to do the calculations and drawing graphs about the outcome. For data analysis test was used by Log Rank Test method Collet, the comparison of survival curves. Statistical analysis can observe the survival curves significantly reduced with the use of diet containing powders Control bees avive until the 21 days, reaching a statistical average of 19 days, and for those treated with 0.25 g, 0.50 g and 1 .0 g respectively experienced mortality at 14, 1 1 and 10 days. The data analysis showed statistically significant differences between treatments and the control, suggesting a toxic effect of the mash obtained from flowers of Jatropha curcas L. to workers of Apis mellifera. As the concentration of macerated white flowers pinion increase in the feed decreases the lifetime of Apis mellifera, showing a toxic effect.