Avaliação do potencial antifúngico do extrato da própolis verde contra leveduras do gênero Candida spp.

Abstract

Candida yeasts reside in the normal microbiota of humans and other animals, but some imbalances can lead to the development of a fungal infection called candidiasis. Moreover, in recent years, resistant strains have been emerging, limiting the therapeutic options for this disease. Thus, the search for new antifungal substances from natural products has been growing, being propolis an excellent example of these products, highlighting their use in treatments of microbial origin. Given the above, the objective was to verify the antifungal activity of green propolis against strains of Candida spp. For this purpose, the Minimum Inhibitory Concentration (MIC) was determined by the microdilution technique, by which the sensitivity of Candida strains to the concentrations of propolis extract in the range of 15,000 μg/mL to 29,3 μg/mL was verified. . MIC values were determined by visual analysis of growth inhibition. Then triphenyltetrazolium chloride (TTC) was added to determine the Minimum Fungicide Concentration (MFC). Both assays were performed in triplicate. MIC results ranged from 117,2 μg/mL to 468,8 μg/mL. CFM results are determined by visualizing the absence of pink/red staining from each well, thus the concentrations obtained were 117,2 μg/mL (Candida albicans HU-01); 468,8 μg / mL (Candida albicans ES-01); 468,8 μg/mL (Candida haemulloni SA-01); 937,5 μg/mL (Candida parapsilosis UR-01). These results attest to the antimicrobial potential of green propolis extract, especially against strains of the genus Candida, contributing to its use as a moderate antimicrobial, especially in infections caused by this oral cavity yeast.