Isolamento e caracterização estrutural de flavonoides de sida santaremnensis h. Monteiro (malvaceae) e avaliação do efeito anti-inflamatório e antitumoral.

Abstract

The phytochemical is dedicated to the structural characterization, evaluation of properties and biosynthetic investigations of natural substances produced by the secondary metabolism of living organisms, which contributes to the dissemination and generation of new knowledge. The Malvaceae family has great economic importance, highlighting in the feed of man and other animals, textile industry, in the production of oils and fiber, in the timber, such as ornamental and medicine. This work aimed to contribute to the phytochemical and pharmacological study of Sida santaremnensis, a species of Malvaceae family, from the isolation, purification and structure determination of chemical constituents of this plant, as well as the evaluation of the immunomodulatory activity and antitumor isolated constituent a majority manner. Through usual chromatographic methods were obtained two flavonoid in Sida santaremnensis: kaempferol and kaempferol 3-O-β-D-glycosyl-6‖-α-L-rhamnoside, both molecules with biological activity evidenced in the literature, the first unprecedented in species. The structural identification of these compounds was carried out using the spectroscopic method of Nuclear Magnetic Resonance Hydrogen and Carbon 13 and comparisons with literature data. The evaluation immunopharmacological developed with kaempferol 3-O-β-D-glycosyl-6 ''-α-L-rhamnoside showed that this presents potential anti-inflammatory action and that is unrelated to the cell death activity. While the evaluation of the antitumor activity demonstrated that this glycosylated flavonoid does not have activity against human promyelocytic leukemia.