Análise da atividade antifúngica de derivados furânicos contra candida spp.

Abstract

The genus Candida represents the main cause of infections of fungal origin. Some species stand out as disease promoters in humans, such as C. albicans, C. glabrata, C. parapsilosis and C. tropicalis. For an evaluation of the antifungal activity performed with the determination of the Minimum Inhibitory Concentration (MIC), the association test with antifungals and verification of the effect of the test tests on the micromorphology of C. albicans, or the effect on the growth kinetics of C albicans and the analysis of ergosterol biosynthesis. As minimal inhibitions of (E) -3- (furan-2-yl) acrylic acid, (E) -3- (furan-2-yl) propyl acrylate, amphotericin B and fluconazole alone against four species of Candida spp varies from 64 to 512 μg / mL, 64 to 512 μg / mL, 1 to 2 μg / mL and 32 to 256 μg / mL, respectively. The synergistic effect of (E) -3- (furan-2-yl) acrylic acid was observed when associated with amphotericin B against C. albicans and C. tropicalis, in relation to (E) -3- (furan-2- il) propyl acrylate, synergistic effect when associated with fluconazole against C. glabrata, there was no antagonism between the substances against any of the tested strains. Propyl (E) -3- (furan-2- yl) acrylic and propyl (E) -3- (furan-2-yl) acrylic promoted morphological changes in C. albicans, decreasing the amount of resistance structures and virulence, such as the formation of pseudo-hyphae, blastoconides and claminoconides, guarantee or antifungal potential of these substances. It was also possible to identify the fungicidal and fungicidal character of (E) -3- (furan-2-yl) acrylic acid and propyl (E) -3- (furan-2-yl) acrylate, respectively, during the study of growth kinetics of C. albicans. Finally, (E) -3- (furan-2-yl) acrylic acid increased the amount of ergosterol in C. albicans and (E) -3- (furan-2-yl) propyl acrylate inhibited the biosynthesis of ergosterol by yeast. Therefore, this study demonstrated great utility in the sense of identifying the antifungal activity of both compounds.