Genética da Intolerância a frutose e a Construção de Primers Específicos para Genes da Enzima Aldolase (ALDOB).

Abstract

Fructose is best known for innate errors associated with its metabolismo that can have important clinical consequences. Thus, there are some pathologies associated whith the consumption of this carbohydrate, onde of which is hereditary fructose intolerance or HFI, a are hereditary pathology, an autosomal recessive silencing disorder due to the absence or deficiency of the enzyme aldolase B in tissues. Thus, the induced fructose is unable to be metabolized based on tissues, causing several potentially fatal symptoms and clinical conditions if they are not treated. In this sense, the acquisition of more information providing about the processes involved with the metabolismo of aldolase can contribute to the development of appropriate Technologies to meet a growing demand in a timely manner in each case, it affections, among intolerances. That said, the present study aims to: analyze the genetics of fructose intolerance and build specific primers for genes of the aldolase Family (ALDOB) important in this process. The methodology applied this work was narrative and with the development of biotechnological and explanatory potential, as well as a bibliographic review as a tool for understanding the effects of genes and de environment in the fructose intolerance, more spacifically on the intolerance process, in addition to to deepen qualitatively the knowledge involving the potentiatities on this issue. The results showed that the kowledge of nutritionists about certain disorders of nutritional origin is still scare due to the huge range of possiblie disorders that exist. Thus, it is noted that it is importante to have a deep clinical look during anamnesis and and na understading of the diferente factors that are behind certain dirsorders such as intolerances. In addition, it was seen that there are three genes for aldolase found in humans responsible for the biochemical pathways of degradation of fructose and mannose borrowed from databases, they are: (ALDOA), (ALDOB), (ALDOC). Several are as bioenergetic statements influenced by these genes. These are expressed differently where any change in the metabolism pathway causes disorders, including fructose intolerance. In conclusion, it was seen that the data obtained in this work are promising for in vitro studies, in addition to being essential for the construction of 10 sets of specific primers for this gene, where 6 are potential uses.