Uso de sensor óptico no estudo de sistemas microfluídicos com, e sem, adsorção biomolecular.

Abstract

This work aims to studymicrofluidic systems, through experimentalmethods, using optical biosensors, more especially, surface plasmons resonance (SPR) technology based sensors. The microfluidic area and biotechnology advancement has enabled the development of microsystems that can potentially be used in public health to supply demands for portable and low cost diagnostic devices. However, microfluidic science still requires greater knowledge of fluid devices properties and performance, when reduced from macro to micro scales. Here a method is proposed for realtime monitoring of SPR surface changes as a novel and important tool of analysis for molecular interaction assays and system performance. On this study one microfluidic cell integrated with a SPR sensor is used. This proposed system enables reduction of samples diffusion time and volume allied with SPR sensor advantages of higher precision and sensitivity. The well defined detection range of refractive index changes, at the SPR sensor surface, guarantees high precision measurements. Here, we perform quantitative assessments of transport parameters, such as slip-flow and convective-diffusion flow velocity as a means to optimize system performance parameters. More specifically, we can evaluate qualitatively and quantitatively the kinetic and thermodynamic properties of biotin and proteins with great reliability. Preliminary immunoassays results allow further development of SPR immunosensors applications for affinity detection in antigen-antibody reactions, with special emphasis on dengue virus (DENV) detection and serotype identification.