Avaliação da atividade antifúngica de KD1 contra Candida spp.

Abstract

Fungal infections have been a worldwide health problem, with an increase in constant in the number of diseases caused by pathogenic fungi. Among the main causes of fungal infections stand out the species of the genus Candida. The treatment pharmacological drug available is still restricted. Triazoles, along with imidazoles, are inserted in the class of azole compounds, the most widely used antifungal agents due to their wide spectrum of activity, effectiveness, and more favorable safety profile. However, the wide application and the emergence of multi-resistant strains culminated in resistance severe to existing antifungals, which significantly reduced its clinical efficacy. This one work aimed to evaluate the antifungal activity of a synthetic substance (KD1) against Candida spp. In this way, tests were performed to determine the Concentration Minimum Inhibitory (MIC) of KD1, against the species C. albicans, C. glabrata, C. krusei, C. tropicalis and C. parapsilosis, obtaining MICs that varied from 32 to 64 μg / mL. In the evaluation micromorphological analysis of KD1 against Candida albicans, C. krusei, C. tropicalis and C. parapsilosis, morphological changes in the normal growth of the fungus have been demonstrated, reducing the proliferation of virulence and resistance factors, such as the development of hyphae, pseudo hyphae, blastoconidia and chlamydocidae. With the Checkerboard method, it was observed indifference, in the concentrations used, of KD1 when associated with amphotericin B, against the species of Candida spp. evaluated. Then he proceeded to the death kinetics test, in that it was possible to observe a strong fungicidal characteristic of KD1, against C. albicans. The substance showed antifungal activity against Candida albicans and non-albicans. Studies of this nature, are extremely relevant, thus increasing the possibilities of pharmacotherapy antifungal.